DNA

Part:BBa_K2119313:Design

Designed by: Macarena Natalia Ramos Briones   Group: iGEM16_Saint_Rose_School_B   (2016-10-14)


Cellobiohydrolase I (Cbh1) promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The big problem by working with this promoter sequence is that all this region of 1500bp contains inducible regions by the clbr gene and repression regions in the presence of glucose. Through bioinformatic software we eliminated this repression regions


Source

this part come from genomic DNA of T. reesei

References